Diagn Microbiol Infect Dis. 2016 Jan 22. pii: S0732-8893(16)00031-6. doi: 10.1016/j.diagmicrobio.2016.01.012. [Epub ahead of print]

MALDI-TOF mass spectrometry for differentiation between Streptococcus pneumoniae and Streptococcus pseudopneumoniae.

van Prehn J1, van Veen SQ2, Schelfaut JJ3, Wessels E3.

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Abstract

We compared the Vitek MS and Microflex MALDI-TOF mass spectrometry platform for species differentiation within the Streptococcus mitis group with PCR assays targeted at lytA, Spn9802, and recA as reference standard. The Vitek MS correctly identified 10/11Streptococcus pneumoniae, 13/13 Streptococcus pseudopneumoniae, and 12/13 S. mitis/oralis. The Microflex correctly identified 9/11 S. pneumoniae, 0/13 S. pseudopneumoniae, and 13/13 S. mitis/oralis. MALDI-TOF is a powerful tool for species determination within the mitis group. Diagnostic accuracy varies depending on platform and database used.

Copyright © 2016 Elsevier Inc. All rights reserved.

KEYWORDS:

Diagnostic accuracy; MALDI-TOF; Mass spectrometry; Mitis group; PCR; Streptococcus

PMID: 26971637 [PubMed - as supplied by publisher]