J Infect Chemother. 2016 Jan 14. pii: S1341-321X(15)00292-5. doi: 10.1016/j.jiac.2015.12.004. [Epub ahead of print]

A novel method for rapid detection of Streptococcus pneumoniae antigens in blood.

Fukushima K1, Kubo T2, Ehara N2, Nakano R2, Matsutake T2, Ishimatu Y3, Tanaka Y4, Akamatsu S4, Izumikawa K3, Kohno S3.

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Abstract

In this study, we used "RAPIRUN®Streptococcus pneumoniae HS (otitis media/sinusitis) (RAPIRUN-HS)," a rapid S. pneumoniae antigen detection kit, to investigate methods for detecting S. pneumoniae antigens in blood of 32 bacterial pneumonia patients. We simultaneously performed PCR to detect S. pneumoniae in blood samples. The results of these tests were compared based on pneumonia severity, determined using the Pneumonia Severity Index (PSI) score classification. Four S. pneumoniae PCR-positive patients of the six severe pneumococcal pneumonia patients (PSI risk class IV/V) also tested positive using RAPIRUN-HS. Twenty-four mild to moderate pneumonia patients (PSI risk class I-III) were S. pneumoniae PCR-negative; of these, 21 tested negative using RAPIRUN-HS. The pneumococcal pneumonia patients testing positive using RAPIRUN-HS had low leukocyte counts and elevated C-reactive protein and procalcitonin levels, indicating that RAPIRUN-HS results were correlated with pneumonia severity. The time course evaluations of the laboratory tests for severe pneumococcal pneumonia patients showed that RAPIRUN-HS and S. pneumoniae PCR yielded positive results earlier than the changes in procalcitonin and IL-6. Thus, concomitant pneumococcal bacteremia was strongly suspected in patients testing positive using RAPIRUN-HS. In conclusion, RAPIRUN-HS may be useful for determining whether to admit patients into hospitals and selecting the appropriate antimicrobial agents.

Copyright © 2015. Published by Elsevier Ltd.

KEYWORDS:

Bacterial pneumonia; Pneumococcal antigen; Pneumonia Severity Index (PSI); RAPIRUN(®)S. pneumoniae HS; Rapid detection kit

PMID: 26778250 [PubMed - as supplied by publisher]