Future Microbiol. 2015 Dec 17. [Epub ahead of print]

Evaluation of phenotypic and molecular methods for identification of Streptococcus pneumoniae.

Yahiaoui RY1,2, den Heijer CD1, Wolfs P1, Bruggeman CA1, Stobberingh EE1,3.

Author information

Abstract

AIM:

The objective of this study is to compare various Streptococcus pneumoniae identification methods.

MATERIALS & METHODS:

In total, 1371 putative S. pneumoniae isolates were tested with three phenotypic methods and a molecular-based method targeting a virulence factor (CpsA). We assessed the sensitivity and the specificity of each method and widely used S. pneumoniae identification algorithm.

RESULTS:

None of the methods or the identification algorithm used separately was able to correctly identify all S. pneumoniae isolates. Furthermore, a high rate of optochin resistance was found.

CONCLUSIONS:

We demonstrated the failure of the current S. pneumoniae identification methods and optochin susceptibility-based algorithm. In addition, the high rate of optochin resistance might justify the necessity of a close monitoring of optochin susceptibility.

KEYWORDS:

PCR; Streptococcus pneumoniae; bile solubility; colony morphology; identification; optochin

PMID: 26673889 [PubMed - as supplied by publisher]