J Microbiol Methods. 2015 Nov 4. pii: S0167-7012(15)30105-6. doi: 10.1016/j.mimet.2015.11.002. [Epub ahead of print]

Capsular typing of streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme.

Ziane H1, Manageiro V2, Ferreira E3, Bektache S1, Tazir M1, Caniça M3.

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Abstract

We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with Quellung reaction.

Copyright © 2015. Published by Elsevier B.V.

KEYWORDS:

Multiplex PCR; Pneumococci; Quellung reaction; Sensitivity; Serogroup/type; Specificity

PMID: 26546733