Inflammation. 2015 Oct 19. [Epub ahead of print]

Initial Host Response to Bacteria in the Murine Lung Differs Between Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus pneumoniae.

Preu L1, Bischoff M2, Veith NT3, Rosenbruch M4, Theegarten D5, Laschke MW6, Meier C1, Tschernig T7.

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Phagocytosis of bacteria is an important process during early host defence. It has been directly observed only ex vivo or in vitro. Here, we report on the observation of phagocytosis under in vivo conditions by using intravital microscopy in the murine lung. Suspensions of fluorescently labelled Streptococcus pneumoniae, Staphylococcus aureus and Pseudomonas aeruginosa cells were each instilled intratracheally to anaesthetized mice. After thoracotomy, the alveolar surface was observed for 30 min. Alveolar phagocytes exhibiting ingested bacteria could be detected and counted. The highest numbers were found after the infection with P. aeruginosa. By using intravital microscopy, cellular host defence could be observed in living mice lungs. The initial phagocytic reaction crucially depends on the species of applied bacteria invading the lung.


Pseudomonas aeruginosa; Staphylococcus aureus; Streptococcus pneumoniae; alveolar phagocytes; host defence; in vivo microscopy; phagocytosis

PMID: 26481126 [PubMed - as supplied by publisher]